Wednesday, November 6, 2019
micro essays a) Fixed smear of Serratia marcescens et al Prepare a fixed smear of Serratia marcescens, et al, using proper aseptic technique. Cover the dried, heat-fixed smear with paper towel. Drop carbolfuchsin onto the paper towel. Steam the smear for five minutes over the slightly boiling water. Let the slide cool for about a minute. Take the paper towel off with forceps and rinse the slide with water. Drop acid-alcohol onto the slide and rinse immediately with distilled water. Rock the smear in acid-alcohol to decolorize it for about 15 seconds. Rinse with distilled water. Add methylene blue to the smear. Wait one minute and then rinse with distilled water. Blot slide and let dry. a) Fixed smear of Bacillus subtillis Prepare a fixed smear of Bacillus subtillis using aseptic technique. Place a piece of paper towel over the dried, heat-fixed smear. Drop the malachite green onto the paper towel. Steam over the slightly boiling water for five minutes. Cool the slide for one minute, remove paper towel, and decolorize using a water rinse from 15 to 30 seconds. Drop safranin onto the smear and wait one minute. Rinse with distilled water. Blot the slide and let dry. After completing the acid-fast stain and the Gram stain, the microbiology student knows how to prepare an acid-fast stain and a Gram stain correctly and aseptically. ...
Posted by Augustine Ahern at 6:58 AM